EQA CD34+

Program description:

The CD34+ EQA is an External Quality Assessment program which allows to control the quantity of antibodies CD34+ staining, RBC lysis on flow cytometry instruments.

The control contains human stem cells and can be used on most of flow cytometers.

Shipments are scheduled twice a year (April and October) with three samples (1,5 ml blood tubes) to obtain 4 evaluation reports over a year.

(control the quantity of antibodies CD34+)

Parameters:

CD34+%: percentage of CD34+

CD34+#: number of CD34+ per microliter

CD45+#: number of CD45+ per microliter

Comparison groups:

All techniques comparison: Statistical treatment made with all the EQA results, all techniques included.

Peer group comparison: Statistical treatment made with the EQA results from laboratories which use the same manufacturer’s cytometer.

We identified 2 peer groups for this program:

• Becton cytometers group

• Coulter cytometers group